This paper is on evolving trends of chatbots on students skills and knowledge acquisition in public universities in Nigeria. University of Port Harcourt River State is among the public universities in Nigeria. Population of this study is 11,000 which comprised 5,000 males and 6,000 female students from University of Port Harcourt River State. Stratified random sampling technique was used to select 35 male respondents in mathematics, educational management and chemistry education departments in the Faculty of Education of University of Port Harcourt. On the other hand, 40 female students were selected from accountancy, Business Administration and Economics departments in the Faculty of management sciences of University of Port Harcourt. This gave a sample size of 225. Descriptive survey research design was used for this study. Two research questions guided this study. Instrument used for data collection was researcher developed questionnaire “titled, evolving trends of chatbots on students skills and knowledge acquistion in public universities in Nigeria” was used, with four rating scale of strongly agree, agree, dis agree and strongly disagree. The instrument was validated by two specialists from Imo State University Owerri. Cronbach alpha statistics was used to determine the reliability coefficient of this study at 0.78 and 0.97, which shows that the instrument was reliable for use in  this study. Mean scores were used to analyze the data. The finding from this study reveals that there are varied uses of chatbots by students in public universities in Nigeria.  Those uses include: Graphic and digital graphic designs, students solutions to their assignments, tailored  information that guide students on  their research among others. In the same vein, challenges that constrain the uses of chatbots among students in public universities in Nigeria via ICT include: Frequent power outage, inability of students to afford ICT some resources and devices, inadequate internet broad band in some public universities in Nigeria. It was recommended in this study that government should provide adequate funding to public higher education institutions in Nigeria. This would enable public universities to install internet facilities, generators to override frequent power outages in public universities in Ngeria.